Construct: shRNA TRCN0000247327

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_026278.3-1175s21c1
DNA Barcode:
GCCAAGCACTACTACTCTAAA

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Lrp2bp (67620)
Gene Description:
Lrp2 binding protein
Transcript:
RefSeq NM_026278.3 (CURRENT)
Match location:
Position 1175 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000247327)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCCAAGCACTACTACTCTAAA
Hairpin Sequence:
5'-CCGG-GCCAAGCACTACTACTCTAAA-CTCGAG-TTTAGAGTAGTAGTGCTTGGC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCCAAGCACTACTACTCTAAACTCGAGTTTAGAGTAGTAGTGCTTGGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCCAAGCACTACTACTCTAAACTCGAGTTTAGAGTAGTAGTGCTTGGC-3'

Other clones with same target sequence:

  1. TRCN0000198288