Construct: shRNA TRCN0000247399

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_172502.3-1471s21c1
DNA Barcode:
ACCAGGTCCTGATCAACATTG

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Dcaf15 (212123)
Gene Description:
DDB1 and CUL4 associated factor 15
Transcript:
RefSeq NM_172502.3 (CURRENT)
Match location:
Position 1471 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000247399)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
ACCAGGTCCTGATCAACATTG
Hairpin Sequence:
5'-CCGG-ACCAGGTCCTGATCAACATTG-CTCGAG-CAATGTTGATCAGGACCTGGT-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGACCAGGTCCTGATCAACATTGCTCGAGCAATGTTGATCAGGACCTGGTTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAACCAGGTCCTGATCAACATTGCTCGAGCAATGTTGATCAGGACCTGGT-3'

Other clones with same target sequence:

(none)