Transcript: Mouse NM_172502.3

Mus musculus DDB1 and CUL4 associated factor 15 (Dcaf15), mRNA.

Source:
NCBI, updated 2017-05-20
Taxon:
Mus musculus (mouse)
Gene:
Dcaf15 (212123)
Length:
2312
CDS:
15..1931

Additional Resources:

NCBI RefSeq record:
NM_172502.3
NBCI Gene record:
Dcaf15 (212123)

sgRNA constructs matching this transcript (CRISPRko, NGG PAM)

This list includes CRISPRko constructs with 100% (20mer + NGG) sequence match to the coding regions of this transcript.

No results found.

shRNA constructs matching this transcript with 100% SDR[?] match

This list includes all shRNAs that have a perfect SDR[?] match to Mouse NM_172502.3, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon).

Clone ID Target Seq Vector Match Position Match Region[?] SDR Match %[?] Intrinsic Score[?] Adjusted Score[?] Matches Other Mouse Gene?[?] Orig. Target Gene[?] Addgene[?]
1 TRCN0000247399 ACCAGGTCCTGATCAACATTG pLKO_005 1471 CDS 100% 10.800 15.120 N Dcaf15 n/a
2 TRCN0000247398 TTACCCTGGACTTCGAGTATG pLKO_005 1351 CDS 100% 10.800 15.120 N Dcaf15 n/a
3 TRCN0000180053 CAACGGGATGCTCATGAACAT pLKO.1 500 CDS 100% 4.950 6.930 N DCAF15 n/a
4 TRCN0000257803 ACGACTTCTCCTTCTACATAT pLKO_005 313 CDS 100% 13.200 10.560 N Dcaf15 n/a
5 TRCN0000189703 GCCTTCCAACTCAAGAAGGAT pLKO.1 714 CDS 100% 0.300 0.240 N Dcaf15 n/a
6 TRCN0000217744 GAGAGTCTGGATGTGTCTTTA pLKO.1 2079 3UTR 100% 13.200 9.240 N Dcaf15 n/a
7 TRCN0000257781 GAGAGTCTGGATGTGTCTTTA pLKO_005 2079 3UTR 100% 13.200 9.240 N Dcaf15 n/a
8 TRCN0000248312 ACCTCAACACAGGCATCTTTG pLKO_005 1579 CDS 100% 10.800 7.560 N Dcaf15 n/a
9 TRCN0000191686 GCTACATCAATTACACCAAGT pLKO.1 1168 CDS 100% 4.050 2.835 N Dcaf15 n/a
10 TRCN0000192773 CAAGGTCATCGTGTTTGGTTT pLKO.1 464 CDS 100% 4.950 2.970 N Dcaf15 n/a
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shRNA constructs with at least a near match to this transcript

This list includes shRNAs that have at least a >84% (16 of 19 bases) SDR[?] match to the transcript NM_172502.3, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon). NOTE: this download is a superset of the above result set.

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All ORF constructs matching this transcript

Clone ID DNA Barcode Vector Sequenced %[?] Nuc. Match %[?] Prot. Match %[?] Epitope Tag Match Diffs[?] Addgene[?]
1 ccsbBroadEn_04513 pDONR223 100% 81% 85.4% None (many diffs) n/a
2 ccsbBroad304_04513 pLX_304 0% 81% 85.4% V5 (many diffs) n/a
3 TRCN0000471829 AGGCCTGTCACTCTTGATGCCAAG pLX_317 23.1% 81% 85.4% V5 (many diffs) n/a
4 ccsbBroadEn_12939 pDONR223 100% 58% 60.1% None (many diffs) n/a
5 ccsbBroad304_12939 pLX_304 0% 58% 60.1% V5 (many diffs) n/a
6 TRCN0000478985 TGCGCGCTCAGAACACTTCTACCC pLX_317 24.4% 58% 60.1% V5 (many diffs) n/a
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