Construct: shRNA TRCN0000247434

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_026017.2-430s21c1
DNA Barcode:
TCAGACTGTTCGATATGATAT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Ctdnep1 (67181)
Gene Description:
CTD nuclear envelope phosphatase 1
Transcript:
RefSeq NM_026017.2 (CURRENT)
Match location:
Position 430 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000247434)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
TCAGACTGTTCGATATGATAT
Hairpin Sequence:
5'-CCGG-TCAGACTGTTCGATATGATAT-CTCGAG-ATATCATATCGAACAGTCTGA-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGTCAGACTGTTCGATATGATATCTCGAGATATCATATCGAACAGTCTGATTTTTG-3'
Reverse sequence:
5'-AATTCAAAAATCAGACTGTTCGATATGATATCTCGAGATATCATATCGAACAGTCTGA-3'

Other clones with same target sequence:

(none)