Construct: shRNA TRCN0000247450

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_028892.4-7158s21c1
DNA Barcode:
GTAGCCTGAATCGGCTTAATA

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Spag17 (74362)
Gene Description:
sperm associated antigen 17
Transcript:
RefSeq NM_028892.4 (CURRENT)
Match location:
Position 7158 (3UTR)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000247450)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GTAGCCTGAATCGGCTTAATA
Hairpin Sequence:
5'-CCGG-GTAGCCTGAATCGGCTTAATA-CTCGAG-TATTAAGCCGATTCAGGCTAC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGTAGCCTGAATCGGCTTAATACTCGAGTATTAAGCCGATTCAGGCTACTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGTAGCCTGAATCGGCTTAATACTCGAGTATTAAGCCGATTCAGGCTAC-3'

Other clones with same target sequence:

(none)