Construct: shRNA TRCN0000247794

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_001037906.2-2603s21c1
DNA Barcode:
TGGGTTGCCTTTACCACTTTG

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Nell1 (338352)
Gene Description:
NEL-like 1
Transcript:
RefSeq NM_001037906.2 (CURRENT)
Match location:
Position 2603 (3UTR)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000247794)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
TGGGTTGCCTTTACCACTTTG
Hairpin Sequence:
5'-CCGG-TGGGTTGCCTTTACCACTTTG-CTCGAG-CAAAGTGGTAAAGGCAACCCA-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGTGGGTTGCCTTTACCACTTTGCTCGAGCAAAGTGGTAAAGGCAACCCATTTTTG-3'
Reverse sequence:
5'-AATTCAAAAATGGGTTGCCTTTACCACTTTGCTCGAGCAAAGTGGTAAAGGCAACCCA-3'

Other clones with same target sequence:

(none)