Transcript: Mouse NM_001037906.2

Mus musculus NEL-like 1 (Nell1), mRNA.

Source:
NCBI, updated 2017-05-28
Taxon:
Mus musculus (mouse)
Gene:
Nell1 (338352)
Length:
2812
CDS:
40..2472

Additional Resources:

NCBI RefSeq record:
NM_001037906.2
NBCI Gene record:
Nell1 (338352)

sgRNA constructs matching this transcript (CRISPRko, NGG PAM)

This list includes CRISPRko constructs with 100% (20mer + NGG) sequence match to the coding regions of this transcript.

No results found.

shRNA constructs matching this transcript with 100% SDR[?] match

This list includes all shRNAs that have a perfect SDR[?] match to Mouse NM_001037906.2, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon).

Clone ID Target Seq Vector Match Position Match Region[?] SDR Match %[?] Intrinsic Score[?] Adjusted Score[?] Matches Other Mouse Gene?[?] Orig. Target Gene[?] Addgene[?]
1 TRCN0000246515 GTCGACTGCAATAGGATTTAT pLKO_005 532 CDS 100% 15.000 21.000 N Nell1 n/a
2 TRCN0000246516 AGAAGAGATACGCTATCATTA pLKO_005 405 CDS 100% 13.200 18.480 N Nell1 n/a
3 TRCN0000247795 CCTGGACAGCTCTGGTATTTC pLKO_005 2340 CDS 100% 13.200 9.240 N Nell1 n/a
4 TRCN0000247796 CTGCTCAGAGAAGGATCATAT pLKO_005 1155 CDS 100% 13.200 9.240 N Nell1 n/a
5 TRCN0000178305 GCAAAGATGCACTACTGTCAT pLKO.1 1357 CDS 100% 4.950 3.465 N Nell1 n/a
6 TRCN0000197466 CAAGGAATAATGGATTTGCAA pLKO.1 751 CDS 100% 3.000 2.100 N Nell1 n/a
7 TRCN0000247794 TGGGTTGCCTTTACCACTTTG pLKO_005 2603 3UTR 100% 10.800 6.480 N Nell1 n/a
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shRNA constructs with at least a near match to this transcript

This list includes shRNAs that have at least a >84% (16 of 19 bases) SDR[?] match to the transcript NM_001037906.2, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon). NOTE: this download is a superset of the above result set.

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All ORF constructs matching this transcript

Clone ID DNA Barcode Vector Sequenced %[?] Nuc. Match %[?] Prot. Match %[?] Epitope Tag Match Diffs[?] Addgene[?]
1 ccsbBroadEn_06631 pDONR223 100% 87.8% 93.2% None (many diffs) n/a
2 ccsbBroad304_06631 pLX_304 0% 87.8% 93.2% V5 (many diffs) n/a
3 TRCN0000473569 CGTTCGCTGCTCCCCGTGCCCCAG pLX_317 19% 87.8% 93.2% V5 (many diffs) n/a
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