Construct: shRNA TRCN0000247927

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_027000.4-1320s21c1
DNA Barcode:
GCCACAATGTTGCTGATTATA

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Gtpbp4 (69237)
Gene Description:
GTP binding protein 4
Transcript:
RefSeq NM_027000.4 (CURRENT)
Match location:
Position 1320 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000247927)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCCACAATGTTGCTGATTATA
Hairpin Sequence:
5'-CCGG-GCCACAATGTTGCTGATTATA-CTCGAG-TATAATCAGCAACATTGTGGC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCCACAATGTTGCTGATTATACTCGAGTATAATCAGCAACATTGTGGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCCACAATGTTGCTGATTATACTCGAGTATAATCAGCAACATTGTGGC-3'

Other clones with same target sequence:

(none)