Construct: shRNA TRCN0000247931

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_027000.4-2230s21c1
DNA Barcode:
TGTCACCTAAAGTACCTATTT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Gtpbp4 (69237)
Gene Description:
GTP binding protein 4
Transcript:
RefSeq NM_027000.4 (CURRENT)
Match location:
Position 2230 (3UTR)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000247931)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
TGTCACCTAAAGTACCTATTT
Hairpin Sequence:
5'-CCGG-TGTCACCTAAAGTACCTATTT-CTCGAG-AAATAGGTACTTTAGGTGACA-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGTGTCACCTAAAGTACCTATTTCTCGAGAAATAGGTACTTTAGGTGACATTTTTG-3'
Reverse sequence:
5'-AATTCAAAAATGTCACCTAAAGTACCTATTTCTCGAGAAATAGGTACTTTAGGTGACA-3'

Other clones with same target sequence:

(none)