Construct: shRNA TRCN0000248035

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_011716.2-1351s21c1
DNA Barcode:
TCCTACTGTCAGTCGTCTTTG

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Wfs1 (22393)
Gene Description:
wolframin ER transmembrane glycoprotein
Transcript:
RefSeq NM_011716.2 (CURRENT)
Match location:
Position 1351 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000248035)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
TCCTACTGTCAGTCGTCTTTG
Hairpin Sequence:
5'-CCGG-TCCTACTGTCAGTCGTCTTTG-CTCGAG-CAAAGACGACTGACAGTAGGA-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGTCCTACTGTCAGTCGTCTTTGCTCGAGCAAAGACGACTGACAGTAGGATTTTTG-3'
Reverse sequence:
5'-AATTCAAAAATCCTACTGTCAGTCGTCTTTGCTCGAGCAAAGACGACTGACAGTAGGA-3'

Other clones with same target sequence:

(none)