Construct: shRNA TRCN0000248039

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_011716.2-883s21c1
DNA Barcode:
AGTACGCCAAGGGCATCATTC

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Wfs1 (22393)
Gene Description:
wolframin ER transmembrane glycoprotein
Transcript:
RefSeq NM_011716.2 (CURRENT)
Match location:
Position 883 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000248039)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
AGTACGCCAAGGGCATCATTC
Hairpin Sequence:
5'-CCGG-AGTACGCCAAGGGCATCATTC-CTCGAG-GAATGATGCCCTTGGCGTACT-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGAGTACGCCAAGGGCATCATTCCTCGAGGAATGATGCCCTTGGCGTACTTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAAGTACGCCAAGGGCATCATTCCTCGAGGAATGATGCCCTTGGCGTACT-3'

Other clones with same target sequence:

(none)