Construct: shRNA TRCN0000248151

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_033602.2-732s21c1
DNA Barcode:
GCCATACACAGCACGCATATT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Peli2 (93834)
Gene Description:
pellino 2
Transcript:
RefSeq NM_033602.2 (CURRENT)
Match location:
Position 732 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000248151)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCCATACACAGCACGCATATT
Hairpin Sequence:
5'-CCGG-GCCATACACAGCACGCATATT-CTCGAG-AATATGCGTGCTGTGTATGGC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCCATACACAGCACGCATATTCTCGAGAATATGCGTGCTGTGTATGGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCCATACACAGCACGCATATTCTCGAGAATATGCGTGCTGTGTATGGC-3'

Other clones with same target sequence:

(none)