Construct: shRNA TRCN0000248283

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_177381.3-3707s21c1
DNA Barcode:
GTTGCTTGGTAGAGCATATTA

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Cog3 (338337)
Gene Description:
component of oligomeric golgi complex 3
Transcript:
RefSeq NM_177381.3 (CURRENT)
Match location:
Position 3707 (3UTR)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000248283)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GTTGCTTGGTAGAGCATATTA
Hairpin Sequence:
5'-CCGG-GTTGCTTGGTAGAGCATATTA-CTCGAG-TAATATGCTCTACCAAGCAAC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGTTGCTTGGTAGAGCATATTACTCGAGTAATATGCTCTACCAAGCAACTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGTTGCTTGGTAGAGCATATTACTCGAGTAATATGCTCTACCAAGCAAC-3'

Other clones with same target sequence:

(none)