Construct: shRNA TRCN0000248318
Construct Description:
- Construct Type:
- shRNA
- Other Identifiers:
- NM_008682.2-379s21c1
- DNA Barcode:
- TGGCAAGTGGAGGCCTAAATA
Original Target:
- Taxon:
-
Mus musculus (mouse)
- Gene:
- Nedd1 (17997)
- Gene Description:
- neural precursor cell expressed, developmentally down-regulated gene 1
- Transcript:
- RefSeq NM_008682.2 (CURRENT)
- Match location:
- Position 379 (CDS)
Vector Information:
- Vector Backbone:
- pLKO_005
- Pol II Cassette 1:
- PGK-PuroR
- Pol II Cassette 2:
- n/a
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Current transcripts matched by this shRNA:
Sequence Information
- Target Sequence:
- TGGCAAGTGGAGGCCTAAATA
- Hairpin Sequence:
-
5'-CCGG-TGGCAAGTGGAGGCCTAAATA-CTCGAG-TATTTAGGCCTCCACTTGCCA-TTTTTG-3'
Oligo design for arrayed cloning:
- Forward sequence:
- 5'-CCGGTGGCAAGTGGAGGCCTAAATACTCGAGTATTTAGGCCTCCACTTGCCATTTTTG-3'
- Reverse sequence:
- 5'-AATTCAAAAATGGCAAGTGGAGGCCTAAATACTCGAGTATTTAGGCCTCCACTTGCCA-3'
Other clones with same target sequence:
(none)
