Construct: shRNA TRCN0000248351

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_138758.1-1252s21c1
DNA Barcode:
AGCCTGGAAAGGTACTATTTA

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Tmlhe (192289)
Gene Description:
trimethyllysine hydroxylase, epsilon
Transcript:
RefSeq NM_138758.1 (CURRENT)
Match location:
Position 1252 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000248351)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
AGCCTGGAAAGGTACTATTTA
Hairpin Sequence:
5'-CCGG-AGCCTGGAAAGGTACTATTTA-CTCGAG-TAAATAGTACCTTTCCAGGCT-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGAGCCTGGAAAGGTACTATTTACTCGAGTAAATAGTACCTTTCCAGGCTTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAAGCCTGGAAAGGTACTATTTACTCGAGTAAATAGTACCTTTCCAGGCT-3'

Other clones with same target sequence:

(none)