Construct: shRNA TRCN0000248597

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_203507.3-793s21c1
DNA Barcode:
TTGACGTCGTGAAGCATTTAA

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Rwdd4a (192174)
Gene Description:
RWD domain containing 4A
Transcript:
RefSeq NM_203507.3 (CURRENT)
Match location:
Position 793 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000248597)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
TTGACGTCGTGAAGCATTTAA
Hairpin Sequence:
5'-CCGG-TTGACGTCGTGAAGCATTTAA-CTCGAG-TTAAATGCTTCACGACGTCAA-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGTTGACGTCGTGAAGCATTTAACTCGAGTTAAATGCTTCACGACGTCAATTTTTG-3'
Reverse sequence:
5'-AATTCAAAAATTGACGTCGTGAAGCATTTAACTCGAGTTAAATGCTTCACGACGTCAA-3'

Other clones with same target sequence:

(none)