Construct: shRNA TRCN0000248615

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_010500.2-978s21c1
DNA Barcode:
TTCTCGGGACTCCTACGGAAA

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Ier5 (15939)
Gene Description:
immediate early response 5
Transcript:
RefSeq NM_010500.2 (CURRENT)
Match location:
Position 978 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000248615)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
TTCTCGGGACTCCTACGGAAA
Hairpin Sequence:
5'-CCGG-TTCTCGGGACTCCTACGGAAA-CTCGAG-TTTCCGTAGGAGTCCCGAGAA-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGTTCTCGGGACTCCTACGGAAACTCGAGTTTCCGTAGGAGTCCCGAGAATTTTTG-3'
Reverse sequence:
5'-AATTCAAAAATTCTCGGGACTCCTACGGAAACTCGAGTTTCCGTAGGAGTCCCGAGAA-3'

Other clones with same target sequence:

(none)