Construct: shRNA TRCN0000248693

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_176849.3-873s21c1
DNA Barcode:
CTGAAGAACAGTTGAGAATTG

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Arglu1 (234023)
Gene Description:
arginine and glutamate rich 1
Transcript:
RefSeq NM_176849.3 (CURRENT)
Match location:
Position 873 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000248693)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CTGAAGAACAGTTGAGAATTG
Hairpin Sequence:
5'-CCGG-CTGAAGAACAGTTGAGAATTG-CTCGAG-CAATTCTCAACTGTTCTTCAG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCTGAAGAACAGTTGAGAATTGCTCGAGCAATTCTCAACTGTTCTTCAGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACTGAAGAACAGTTGAGAATTGCTCGAGCAATTCTCAACTGTTCTTCAG-3'

Other clones with same target sequence:

(none)