Construct: shRNA TRCN0000249002

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_053255.3-1021s21c1
DNA Barcode:
ACTTCAGTCAGAGGTACAAAC

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Elac1 (114615)
Gene Description:
elaC ribonuclease Z 1
Transcript:
RefSeq NM_053255.3 (CURRENT)
Match location:
Position 1021 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000249002)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
ACTTCAGTCAGAGGTACAAAC
Hairpin Sequence:
5'-CCGG-ACTTCAGTCAGAGGTACAAAC-CTCGAG-GTTTGTACCTCTGACTGAAGT-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGACTTCAGTCAGAGGTACAAACCTCGAGGTTTGTACCTCTGACTGAAGTTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAACTTCAGTCAGAGGTACAAACCTCGAGGTTTGTACCTCTGACTGAAGT-3'

Other clones with same target sequence:

(none)