Construct: shRNA TRCN0000249719

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_008889.2-407s21c1
DNA Barcode:
TCAACCTGGAGGAGTGGATTT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Ppp1r14b (18938)
Gene Description:
protein phosphatase 1, regulatory (inhibitor) subunit 14B
Transcript:
RefSeq NM_008889.2 (CURRENT)
Match location:
Position 407 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000249719)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
TCAACCTGGAGGAGTGGATTT
Hairpin Sequence:
5'-CCGG-TCAACCTGGAGGAGTGGATTT-CTCGAG-AAATCCACTCCTCCAGGTTGA-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGTCAACCTGGAGGAGTGGATTTCTCGAGAAATCCACTCCTCCAGGTTGATTTTTG-3'
Reverse sequence:
5'-AATTCAAAAATCAACCTGGAGGAGTGGATTTCTCGAGAAATCCACTCCTCCAGGTTGA-3'

Other clones with same target sequence:

(none)