Construct: shRNA TRCN0000249867

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_010244.3-73s21c1
DNA Barcode:
GATAATGACTCTCCGGATAAT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Fv1 (14349)
Gene Description:
Friend virus susceptibility 1
Transcript:
RefSeq NM_010244.3 (CURRENT)
Match location:
Position 73 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000249867)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GATAATGACTCTCCGGATAAT
Hairpin Sequence:
5'-CCGG-GATAATGACTCTCCGGATAAT-CTCGAG-ATTATCCGGAGAGTCATTATC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGATAATGACTCTCCGGATAATCTCGAGATTATCCGGAGAGTCATTATCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGATAATGACTCTCCGGATAATCTCGAGATTATCCGGAGAGTCATTATC-3'

Other clones with same target sequence:

(none)