Construct: shRNA TRCN0000250686

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_172770.3-1887s21c1
DNA Barcode:
GAAGTAGTGAGACTTGATAAA

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Ttc12 (235330)
Gene Description:
tetratricopeptide repeat domain 12
Transcript:
RefSeq NM_172770.3 (CURRENT)
Match location:
Position 1887 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000250686)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GAAGTAGTGAGACTTGATAAA
Hairpin Sequence:
5'-CCGG-GAAGTAGTGAGACTTGATAAA-CTCGAG-TTTATCAAGTCTCACTACTTC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGAAGTAGTGAGACTTGATAAACTCGAGTTTATCAAGTCTCACTACTTCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGAAGTAGTGAGACTTGATAAACTCGAGTTTATCAAGTCTCACTACTTC-3'

Other clones with same target sequence:

(none)