Construct: shRNA TRCN0000250880

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_019953.1-803s21c1
DNA Barcode:
GAGAGGCTGACAACGTTAAAG

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Cnpy2 (56530)
Gene Description:
canopy FGF signaling regulator 2
Transcript:
RefSeq NM_019953.1 (CURRENT)
Match location:
Position 803 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000250880)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GAGAGGCTGACAACGTTAAAG
Hairpin Sequence:
5'-CCGG-GAGAGGCTGACAACGTTAAAG-CTCGAG-CTTTAACGTTGTCAGCCTCTC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGAGAGGCTGACAACGTTAAAGCTCGAGCTTTAACGTTGTCAGCCTCTCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGAGAGGCTGACAACGTTAAAGCTCGAGCTTTAACGTTGTCAGCCTCTC-3'

Other clones with same target sequence:

(none)