Construct: shRNA TRCN0000250986

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_027950.1-451s21c1
DNA Barcode:
TGCGTCCTGACACAGACTTTG

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Osgin1 (71839)
Gene Description:
oxidative stress induced growth inhibitor 1
Transcript:
RefSeq NM_027950.1 (CURRENT)
Match location:
Position 451 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000250986)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
TGCGTCCTGACACAGACTTTG
Hairpin Sequence:
5'-CCGG-TGCGTCCTGACACAGACTTTG-CTCGAG-CAAAGTCTGTGTCAGGACGCA-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGTGCGTCCTGACACAGACTTTGCTCGAGCAAAGTCTGTGTCAGGACGCATTTTTG-3'
Reverse sequence:
5'-AATTCAAAAATGCGTCCTGACACAGACTTTGCTCGAGCAAAGTCTGTGTCAGGACGCA-3'

Other clones with same target sequence:

(none)