Construct: shRNA TRCN0000251084

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_177047.3-2480s21c1
DNA Barcode:
CATATCGCCTGGCAGATTTAT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Auts2 (319974)
Gene Description:
autism susceptibility candidate 2
Transcript:
RefSeq NM_177047.3 (CURRENT)
Match location:
Position 2480 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000251084)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CATATCGCCTGGCAGATTTAT
Hairpin Sequence:
5'-CCGG-CATATCGCCTGGCAGATTTAT-CTCGAG-ATAAATCTGCCAGGCGATATG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCATATCGCCTGGCAGATTTATCTCGAGATAAATCTGCCAGGCGATATGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACATATCGCCTGGCAGATTTATCTCGAGATAAATCTGCCAGGCGATATG-3'

Other clones with same target sequence:

(none)