Construct: shRNA TRCN0000251142

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_029896.1-753s21c1
DNA Barcode:
TTTGATCCAGAAGGGTTAATT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Wdr82 (77305)
Gene Description:
WD repeat domain containing 82
Transcript:
RefSeq NM_029896.1 (CURRENT)
Match location:
Position 753 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000251142)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
TTTGATCCAGAAGGGTTAATT
Hairpin Sequence:
5'-CCGG-TTTGATCCAGAAGGGTTAATT-CTCGAG-AATTAACCCTTCTGGATCAAA-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGTTTGATCCAGAAGGGTTAATTCTCGAGAATTAACCCTTCTGGATCAAATTTTTG-3'
Reverse sequence:
5'-AATTCAAAAATTTGATCCAGAAGGGTTAATTCTCGAGAATTAACCCTTCTGGATCAAA-3'

Other clones with same target sequence:

  1. TRCN0000330747