Construct: shRNA TRCN0000251290

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_001081281.1-247s21c1
DNA Barcode:
TCACGAAGCCTGTGGTCATTC

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Trim55 (381485)
Gene Description:
tripartite motif-containing 55
Transcript:
RefSeq NM_001081281.1 (CURRENT)
Match location:
Position 247 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000251290)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
TCACGAAGCCTGTGGTCATTC
Hairpin Sequence:
5'-CCGG-TCACGAAGCCTGTGGTCATTC-CTCGAG-GAATGACCACAGGCTTCGTGA-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGTCACGAAGCCTGTGGTCATTCCTCGAGGAATGACCACAGGCTTCGTGATTTTTG-3'
Reverse sequence:
5'-AATTCAAAAATCACGAAGCCTGTGGTCATTCCTCGAGGAATGACCACAGGCTTCGTGA-3'

Other clones with same target sequence:

(none)