Transcript: Mouse NM_001081281.1

Mus musculus tripartite motif-containing 55 (Trim55), mRNA.

Source:
NCBI, updated 2017-06-25
Taxon:
Mus musculus (mouse)
Gene:
Trim55 (381485)
Length:
1787
CDS:
150..1787

Additional Resources:

NCBI RefSeq record:
NM_001081281.1
NBCI Gene record:
Trim55 (381485)

sgRNA constructs matching this transcript (CRISPRko, NGG PAM)

This list includes CRISPRko constructs with 100% (20mer + NGG) sequence match to the coding regions of this transcript.

No results found.

shRNA constructs matching this transcript with 100% SDR[?] match

This list includes all shRNAs that have a perfect SDR[?] match to Mouse NM_001081281.1, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon).

Clone ID Target Seq Vector Match Position Match Region[?] SDR Match %[?] Intrinsic Score[?] Adjusted Score[?] Matches Other Mouse Gene?[?] Orig. Target Gene[?] Addgene[?]
1 TRCN0000251293 AGGCCTCTAACCCGTACTTAC pLKO_005 316 CDS 100% 10.800 15.120 N Trim55 n/a
2 TRCN0000251289 TTCATGGATGAGCCCGAAATG pLKO_005 951 CDS 100% 10.800 8.640 N Trim55 n/a
3 TRCN0000251292 TTTACCCTAGTTGGTATAAAG pLKO_005 1462 CDS 100% 13.200 9.240 N Trim55 n/a
4 TRCN0000251291 CAGAGCTCAGTGATGGTATTG pLKO_005 658 CDS 100% 10.800 7.560 N Trim55 n/a
5 TRCN0000251290 TCACGAAGCCTGTGGTCATTC pLKO_005 247 CDS 100% 10.800 7.560 N Trim55 n/a
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shRNA constructs with at least a near match to this transcript

This list includes shRNAs that have at least a >84% (16 of 19 bases) SDR[?] match to the transcript NM_001081281.1, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon). NOTE: this download is a superset of the above result set.

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All ORF constructs matching this transcript

Clone ID DNA Barcode Vector Sequenced %[?] Nuc. Match %[?] Prot. Match %[?] Epitope Tag Match Diffs[?] Addgene[?]
1 ccsbBroadEn_04413 pDONR223 100% 69.9% 71.4% None (many diffs) n/a
2 ccsbBroad304_04413 pLX_304 0% 69.9% 71.4% V5 (many diffs) n/a
3 TRCN0000473514 CAGTGGGAGGCAACCACGCAGACC pLX_317 36.4% 69.9% 71.4% V5 (many diffs) n/a
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