Construct: shRNA TRCN0000251401

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_001109985.1-2790s21c1
DNA Barcode:
CCTGCAACCCAGCTCATATAA

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Nos1ap (70729)
Gene Description:
nitric oxide synthase 1 (neuronal) adaptor protein
Transcript:
RefSeq NM_001109985.1 (CURRENT)
Match location:
Position 2790 (3UTR)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000251401)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CCTGCAACCCAGCTCATATAA
Hairpin Sequence:
5'-CCGG-CCTGCAACCCAGCTCATATAA-CTCGAG-TTATATGAGCTGGGTTGCAGG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCCTGCAACCCAGCTCATATAACTCGAGTTATATGAGCTGGGTTGCAGGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACCTGCAACCCAGCTCATATAACTCGAGTTATATGAGCTGGGTTGCAGG-3'

Other clones with same target sequence:

(none)