Construct: shRNA TRCN0000251558

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_134126.3-3020s21c1
DNA Barcode:
ATGGAAGTGCCTCACTATTAC

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Ift140 (106633)
Gene Description:
intraflagellar transport 140
Transcript:
RefSeq NM_134126.3 (CURRENT)
Match location:
Position 3020 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000251558)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
ATGGAAGTGCCTCACTATTAC
Hairpin Sequence:
5'-CCGG-ATGGAAGTGCCTCACTATTAC-CTCGAG-GTAATAGTGAGGCACTTCCAT-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGATGGAAGTGCCTCACTATTACCTCGAGGTAATAGTGAGGCACTTCCATTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAATGGAAGTGCCTCACTATTACCTCGAGGTAATAGTGAGGCACTTCCAT-3'

Other clones with same target sequence:

(none)