Construct: shRNA TRCN0000251576

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_001081421.1-1584s21c1
DNA Barcode:
CCAGCCCAGGCATGGTTATTT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Galnt16 (108760)
Gene Description:
UDP-N-acetyl-alpha-D-galactosamine:polypeptide N-acetylgalactosaminyltransferase 16
Transcript:
RefSeq NM_001081421.1 (CURRENT)
Match location:
Position 1584 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000251576)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CCAGCCCAGGCATGGTTATTT
Hairpin Sequence:
5'-CCGG-CCAGCCCAGGCATGGTTATTT-CTCGAG-AAATAACCATGCCTGGGCTGG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCCAGCCCAGGCATGGTTATTTCTCGAGAAATAACCATGCCTGGGCTGGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACCAGCCCAGGCATGGTTATTTCTCGAGAAATAACCATGCCTGGGCTGG-3'

Other clones with same target sequence:

(none)