Construct: shRNA TRCN0000251640

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_026001.2-61s21c1
DNA Barcode:
TCCTACTTCCAGAACATTTAA

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Rnaseh2b (67153)
Gene Description:
ribonuclease H2, subunit B
Transcript:
RefSeq NM_026001.2 (CURRENT)
Match location:
Position 61 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000251640)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
TCCTACTTCCAGAACATTTAA
Hairpin Sequence:
5'-CCGG-TCCTACTTCCAGAACATTTAA-CTCGAG-TTAAATGTTCTGGAAGTAGGA-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGTCCTACTTCCAGAACATTTAACTCGAGTTAAATGTTCTGGAAGTAGGATTTTTG-3'
Reverse sequence:
5'-AATTCAAAAATCCTACTTCCAGAACATTTAACTCGAGTTAAATGTTCTGGAAGTAGGA-3'

Other clones with same target sequence:

(none)