Construct: shRNA TRCN0000251710

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_001081060.1-1494s21c1
DNA Barcode:
CAGAGATAAGTGGTCCAATTT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Slc9a3 (105243)
Gene Description:
solute carrier family 9 (sodium/hydrogen exchanger), member 3
Transcript:
RefSeq NM_001081060.1 (CURRENT)
Match location:
Position 1494 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000251710)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CAGAGATAAGTGGTCCAATTT
Hairpin Sequence:
5'-CCGG-CAGAGATAAGTGGTCCAATTT-CTCGAG-AAATTGGACCACTTATCTCTG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCAGAGATAAGTGGTCCAATTTCTCGAGAAATTGGACCACTTATCTCTGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACAGAGATAAGTGGTCCAATTTCTCGAGAAATTGGACCACTTATCTCTG-3'

Other clones with same target sequence:

(none)