Construct: shRNA TRCN0000251748

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_172409.2-2853s21c1
DNA Barcode:
CAAACACTGTTGCACTATATA

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Fmnl2 (71409)
Gene Description:
formin-like 2
Transcript:
RefSeq NM_172409.2 (CURRENT)
Match location:
Position 2853 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000251748)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CAAACACTGTTGCACTATATA
Hairpin Sequence:
5'-CCGG-CAAACACTGTTGCACTATATA-CTCGAG-TATATAGTGCAACAGTGTTTG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCAAACACTGTTGCACTATATACTCGAGTATATAGTGCAACAGTGTTTGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACAAACACTGTTGCACTATATACTCGAGTATATAGTGCAACAGTGTTTG-3'

Other clones with same target sequence:

  1. TRCN0000369345