Construct: shRNA TRCN0000251833

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_001044380.1-1622s21c1
DNA Barcode:
ATCTCAATGGCCTCTACATTT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Hhipl1 (214305)
Gene Description:
hedgehog interacting protein-like 1
Transcript:
RefSeq NM_001044380.1 (CURRENT)
Match location:
Position 1622 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000251833)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
ATCTCAATGGCCTCTACATTT
Hairpin Sequence:
5'-CCGG-ATCTCAATGGCCTCTACATTT-CTCGAG-AAATGTAGAGGCCATTGAGAT-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGATCTCAATGGCCTCTACATTTCTCGAGAAATGTAGAGGCCATTGAGATTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAATCTCAATGGCCTCTACATTTCTCGAGAAATGTAGAGGCCATTGAGAT-3'

Other clones with same target sequence:

(none)