Construct: shRNA TRCN0000251840

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_001034858.3-2611s21c1
DNA Barcode:
CTCATCCTCTTGTCGACATTT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Armc2 (213402)
Gene Description:
armadillo repeat containing 2
Transcript:
RefSeq NM_001034858.3 (CURRENT)
Match location:
Position 2611 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000251840)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CTCATCCTCTTGTCGACATTT
Hairpin Sequence:
5'-CCGG-CTCATCCTCTTGTCGACATTT-CTCGAG-AAATGTCGACAAGAGGATGAG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCTCATCCTCTTGTCGACATTTCTCGAGAAATGTCGACAAGAGGATGAGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACTCATCCTCTTGTCGACATTTCTCGAGAAATGTCGACAAGAGGATGAG-3'

Other clones with same target sequence:

(none)