Construct: shRNA TRCN0000252105

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_001002900.1-311s21c1
DNA Barcode:
GACCTCGGTTCTTCAATGTAC

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Higd1c (380975)
Gene Description:
HIG1 domain family, member 1C
Transcript:
RefSeq NM_001002900.1 (CURRENT)
Match location:
Position 311 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000252105)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GACCTCGGTTCTTCAATGTAC
Hairpin Sequence:
5'-CCGG-GACCTCGGTTCTTCAATGTAC-CTCGAG-GTACATTGAAGAACCGAGGTC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGACCTCGGTTCTTCAATGTACCTCGAGGTACATTGAAGAACCGAGGTCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGACCTCGGTTCTTCAATGTACCTCGAGGTACATTGAAGAACCGAGGTC-3'

Other clones with same target sequence:

(none)