Construct: shRNA TRCN0000252179

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_028854.3-687s21c1
DNA Barcode:
ACTCTACTGGACCCTGAATTT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Tbc1d21 (74286)
Gene Description:
TBC1 domain family, member 21
Transcript:
RefSeq NM_028854.3 (CURRENT)
Match location:
Position 687 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000252179)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
ACTCTACTGGACCCTGAATTT
Hairpin Sequence:
5'-CCGG-ACTCTACTGGACCCTGAATTT-CTCGAG-AAATTCAGGGTCCAGTAGAGT-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGACTCTACTGGACCCTGAATTTCTCGAGAAATTCAGGGTCCAGTAGAGTTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAACTCTACTGGACCCTGAATTTCTCGAGAAATTCAGGGTCCAGTAGAGT-3'

Other clones with same target sequence:

(none)