Construct: shRNA TRCN0000252197

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_001037744.1-299s21c1
DNA Barcode:
TCCGACTGATCTCAGCGTTTC

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Timm8a2 (223262)
Gene Description:
translocase of inner mitochondrial membrane 8A2
Transcript:
RefSeq NM_001037744.1 (CURRENT)
Match location:
Position 299 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000252197)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
TCCGACTGATCTCAGCGTTTC
Hairpin Sequence:
5'-CCGG-TCCGACTGATCTCAGCGTTTC-CTCGAG-GAAACGCTGAGATCAGTCGGA-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGTCCGACTGATCTCAGCGTTTCCTCGAGGAAACGCTGAGATCAGTCGGATTTTTG-3'
Reverse sequence:
5'-AATTCAAAAATCCGACTGATCTCAGCGTTTCCTCGAGGAAACGCTGAGATCAGTCGGA-3'

Other clones with same target sequence:

(none)