Construct: shRNA TRCN0000252198

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_001037744.1-228s21c1
DNA Barcode:
ATACGAGCCAGTTCATCTTGA

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Timm8a2 (223262)
Gene Description:
translocase of inner mitochondrial membrane 8A2
Transcript:
RefSeq NM_001037744.1 (CURRENT)
Match location:
Position 228 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000252198)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
ATACGAGCCAGTTCATCTTGA
Hairpin Sequence:
5'-CCGG-ATACGAGCCAGTTCATCTTGA-CTCGAG-TCAAGATGAACTGGCTCGTAT-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGATACGAGCCAGTTCATCTTGACTCGAGTCAAGATGAACTGGCTCGTATTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAATACGAGCCAGTTCATCTTGACTCGAGTCAAGATGAACTGGCTCGTAT-3'

Other clones with same target sequence:

(none)