Construct: shRNA TRCN0000252203

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_001001334.2-156s21c1
DNA Barcode:
ACTGCAGAATGCGGGTATAAT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Spag6 (381350)
Gene Description:
sperm associated antigen 6
Transcript:
RefSeq NM_001001334.2 (CURRENT)
Match location:
Position 156 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000252203)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
ACTGCAGAATGCGGGTATAAT
Hairpin Sequence:
5'-CCGG-ACTGCAGAATGCGGGTATAAT-CTCGAG-ATTATACCCGCATTCTGCAGT-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGACTGCAGAATGCGGGTATAATCTCGAGATTATACCCGCATTCTGCAGTTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAACTGCAGAATGCGGGTATAATCTCGAGATTATACCCGCATTCTGCAGT-3'

Other clones with same target sequence:

(none)