Construct: shRNA TRCN0000252364

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_001080931.1-2260s21c1
DNA Barcode:
GATGCTCCACGCCCTACAAAT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Med13 (327987)
Gene Description:
mediator complex subunit 13
Transcript:
RefSeq NM_001080931.1 (CURRENT)
Match location:
Position 2260 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000252364)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GATGCTCCACGCCCTACAAAT
Hairpin Sequence:
5'-CCGG-GATGCTCCACGCCCTACAAAT-CTCGAG-ATTTGTAGGGCGTGGAGCATC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGATGCTCCACGCCCTACAAATCTCGAGATTTGTAGGGCGTGGAGCATCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGATGCTCCACGCCCTACAAATCTCGAGATTTGTAGGGCGTGGAGCATC-3'

Other clones with same target sequence:

(none)