Construct: shRNA TRCN0000252370

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_001080769.1-2172s21c1
DNA Barcode:
GTTGTAGCTACGGTCACTTTC

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Uhrf1bp1 (224648)
Gene Description:
UHRF1 (ICBP90) binding protein 1
Transcript:
RefSeq NM_001080769.1 (CURRENT)
Match location:
Position 2172 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000252370)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GTTGTAGCTACGGTCACTTTC
Hairpin Sequence:
5'-CCGG-GTTGTAGCTACGGTCACTTTC-CTCGAG-GAAAGTGACCGTAGCTACAAC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGTTGTAGCTACGGTCACTTTCCTCGAGGAAAGTGACCGTAGCTACAACTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGTTGTAGCTACGGTCACTTTCCTCGAGGAAAGTGACCGTAGCTACAAC-3'

Other clones with same target sequence:

(none)