Construct: shRNA TRCN0000252557

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_001081418.1-4631s21c1
DNA Barcode:
CCCGCAGGATGATACACTTAC

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Bicra (243842)
Gene Description:
BRD4 interacting chromatin remodeling complex associated protein
Transcript:
RefSeq NM_001081418.1 (CURRENT)
Match location:
Position 4631 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000252557)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CCCGCAGGATGATACACTTAC
Hairpin Sequence:
5'-CCGG-CCCGCAGGATGATACACTTAC-CTCGAG-GTAAGTGTATCATCCTGCGGG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCCCGCAGGATGATACACTTACCTCGAGGTAAGTGTATCATCCTGCGGGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACCCGCAGGATGATACACTTACCTCGAGGTAAGTGTATCATCCTGCGGG-3'

Other clones with same target sequence:

(none)