Construct: shRNA TRCN0000252626

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_001111277.1-1150s21c1
DNA Barcode:
CATCGGGCCAGATACACAAAT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Eif2b3 (108067)
Gene Description:
eukaryotic translation initiation factor 2B, subunit 3
Transcript:
RefSeq NM_001111277.1 (CURRENT)
Match location:
Position 1150 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000252626)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CATCGGGCCAGATACACAAAT
Hairpin Sequence:
5'-CCGG-CATCGGGCCAGATACACAAAT-CTCGAG-ATTTGTGTATCTGGCCCGATG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCATCGGGCCAGATACACAAATCTCGAGATTTGTGTATCTGGCCCGATGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACATCGGGCCAGATACACAAATCTCGAGATTTGTGTATCTGGCCCGATG-3'

Other clones with same target sequence:

(none)