Construct: shRNA TRCN0000252861

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_023623.2-205s21c1
DNA Barcode:
GTGAAGATGTTCCCAATTGTC

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Cyp2d40 (71754)
Gene Description:
cytochrome P450, family 2, subfamily d, polypeptide 40
Transcript:
RefSeq NM_023623.2 (CURRENT)
Match location:
Position 205 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000252861)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GTGAAGATGTTCCCAATTGTC
Hairpin Sequence:
5'-CCGG-GTGAAGATGTTCCCAATTGTC-CTCGAG-GACAATTGGGAACATCTTCAC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGTGAAGATGTTCCCAATTGTCCTCGAGGACAATTGGGAACATCTTCACTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGTGAAGATGTTCCCAATTGTCCTCGAGGACAATTGGGAACATCTTCAC-3'

Other clones with same target sequence:

(none)