Construct: shRNA TRCN0000253206

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_001100183.1-1153s21c1
DNA Barcode:
ATACCATCTGTTGGCAGAAAG

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Cyp4a29 (230639)
Gene Description:
cytochrome P450, family 4, subfamily a, polypeptide 29
Transcript:
RefSeq NM_001100183.1 (CURRENT)
Match location:
Position 1153 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000253206)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
ATACCATCTGTTGGCAGAAAG
Hairpin Sequence:
5'-CCGG-ATACCATCTGTTGGCAGAAAG-CTCGAG-CTTTCTGCCAACAGATGGTAT-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGATACCATCTGTTGGCAGAAAGCTCGAGCTTTCTGCCAACAGATGGTATTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAATACCATCTGTTGGCAGAAAGCTCGAGCTTTCTGCCAACAGATGGTAT-3'

Other clones with same target sequence:

(none)