Construct: shRNA TRCN0000253392

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_001081007.1-467s21c1
DNA Barcode:
GCCTCCTAAATCGATCGTAAT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Zfp382 (233060)
Gene Description:
zinc finger protein 382
Transcript:
RefSeq NM_001081007.1 (CURRENT)
Match location:
Position 467 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000253392)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCCTCCTAAATCGATCGTAAT
Hairpin Sequence:
5'-CCGG-GCCTCCTAAATCGATCGTAAT-CTCGAG-ATTACGATCGATTTAGGAGGC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCCTCCTAAATCGATCGTAATCTCGAGATTACGATCGATTTAGGAGGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCCTCCTAAATCGATCGTAATCTCGAGATTACGATCGATTTAGGAGGC-3'

Other clones with same target sequence:

(none)