Construct: shRNA TRCN0000253467

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_001029878.1-802s21c1
DNA Barcode:
CTGACTGAAGAGCTGATATTC

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Lonrf2 (381338)
Gene Description:
LON peptidase N-terminal domain and ring finger 2
Transcript:
RefSeq NM_001029878.1 (CURRENT)
Match location:
Position 802 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000253467)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CTGACTGAAGAGCTGATATTC
Hairpin Sequence:
5'-CCGG-CTGACTGAAGAGCTGATATTC-CTCGAG-GAATATCAGCTCTTCAGTCAG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCTGACTGAAGAGCTGATATTCCTCGAGGAATATCAGCTCTTCAGTCAGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACTGACTGAAGAGCTGATATTCCTCGAGGAATATCAGCTCTTCAGTCAG-3'

Other clones with same target sequence:

(none)