Construct: shRNA TRCN0000253535

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_172674.2-2701s21c1
DNA Barcode:
CAGAAACCTCGCGCCTATTAT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Phf20 (228829)
Gene Description:
PHD finger protein 20
Transcript:
RefSeq NM_172674.2 (CURRENT)
Match location:
Position 2701 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000253535)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CAGAAACCTCGCGCCTATTAT
Hairpin Sequence:
5'-CCGG-CAGAAACCTCGCGCCTATTAT-CTCGAG-ATAATAGGCGCGAGGTTTCTG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCAGAAACCTCGCGCCTATTATCTCGAGATAATAGGCGCGAGGTTTCTGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACAGAAACCTCGCGCCTATTATCTCGAGATAATAGGCGCGAGGTTTCTG-3'

Other clones with same target sequence:

(none)